In style, her paintings resemble comic-book serials, but they also contain stylistic references to hieroglyphics and American folk-art traditions. Ali often achieves a high level of emotional tension in her work as a result of juxtaposing brightly colored scenes with dark, often violent subject matter that speaks of political resistance, social relationships, and betrayal. Although Ali’s interest in representations of socio-political issues and current events drives her work, her finished paintings rarely reveal specific references.

Her most famous and longest-running series of paintings depicts the brown-skinned and gender-neutral Greenheads, while her most recent works include portraits as well as more abstract biomorphic images. Ali endows the characters and scenes in her paintings with everyday attributes like dodge balls, sneakers, and Band-aids, as well as historically- and culturally-loaded items such as nooses, hoods, robes, masks, and military-style uniforms. Her drawings, which she describes as “automatic,” are looser and more playful than the paintings and are often the source of material that she explores more deeply in her paintings.

Laylah Ali has had solo exhibitions at the Museum of Modern Art, New York; the Institute of Contemporary Art, Boston; Museum of Contemporary Art, Chicago; Contemporary Art Museum, St. Louis; and Massachusetts Museum of Contemporary Art; among others. Her work was exhibited at the Venice Biennale (2003) and the Whitney Biennial (2004).